An international assembly of researchers from the United States Food and Drug Administration, the Finland National Institute for Health and Welfare, and World Health Organization Regional Laboratory at the Pakistan National Institute of Health published a paper in the April 2016 issue of Eurosurveillance entitled “Environmental Surveillance of Viruses by Tangential Flow Filtration and Metagenomic Reconstruction”. The focus of the article was to sequence poliovirus through the collection of sewage samples.
The investigators filtered sewage samples from Finland and Pakistan through a 0.2 μm MidiKros® and then concentrated the samples using the KR2i and 100 kDa MidiKros® filters.
Abstract: An approach is proposed for environmental surveillance of poliovirus by concentrating sewage samples with tangential flow filtration (TFF) followed by deep sequencing of viral RNA. Subsequent to testing the method with samples from Finland, samples from Pakistan, a country endemic for poliovirus, were investigated. Click here to read the article.
The scientists determined that TFF more effectively concentrated the viruses than traditional methods that require toxic organic solvents. The procedure presented in article could prove useful for environmental surveillance of other viruses.
Tangential Flow Filtration (TFF) also known as Cross Flow Filtration involves the recirculation of the retentate across the surface of the membrane. This gentle cross flow feed acts to minimize membrane fouling, maintain a high filtration rate and provide higher product recovery. Click here for more information.