Scientists with The University of Texas MD Anderson Cancer Center, Department of Chemical and Biomolecular Engineering at the University of Houston, and the Tulane University Health Science Center published an article in the December 2014 volume of Journal of Chromatography A titled “Benchtop isolation and characterization of functional exosomes by sequential filtration”. This paper proposed a 3-step process for exosome isolation, a method that could prove useful for clinical applications.
One step of their 3-part process is to use our our KR2i TFF System and a 500 kDa mPES MidiKros® to concentrate the molecules to a volume of 50 ml.
Abstract: Early and minimally invasive detection of malignant events or other pathologies is of utmost importance in the pursuit of improved patient care and outcomes. Recent evidence indicates that exosomes and extracellular vesicles in serum and body fluids can contain nucleic acid, protein, and other biomarkers. Accordingly, there is great interest in applying these clinically as prognostic, predictive, pharmacodynamic, and early detection indicators.
Modified Polyethersulfone (mPES) is an advanced hydrophilic membrane filtration chemistry that provides higher flux rates for faster processing times, excellent selectivity for separation applications, and low protein binding for higher product yields than other membrane chemistries. Click here for more on mPES.
SpectrumLabs.com recently launched our second generation KR2i TFF System. The KR2i is ideal for small volume, R&D scale microfiltration and ultrafiltration, with processing volume ranges from 1 milliliter to 10 liters.