Sanofi Pasteur published “Preparation of pure, high titer, pseudoinfectious Flavivirus particles by hollow fiber tangential flow filtration and anion exchange chromatography” in the August 2015 issue of Vaccine. This paper highlights our MidiKros® and KR2i TFF System in the concentration and purification step of their process.

As the researchers from Sanofi Pasteur explain in Discussions and Conclusions (item 4), they used our hollow fiber TFF system rather than a flat sheet membrabe system because of “shear sensitivity when purifying infectious substances”. They go on to say that TFF is superior for purification of infectious viruses.

Abstract: Purification of enveloped viruses such as live flavivirus vaccine candidates poses a challenge as one must retain viral infectivity to preserve immunogenicity. Here we describe a laboratory-scale purification procedure for two replication defective (single-cycle) flavivirus variants for use in a pre-clinical setting. The two step purification scheme based on hollow fiber tangential flow filtration (TFF) followed by anion exchange chromatography… Click here to read more.


Tangential Flow Filtration (TFF), also known as Cross Flow Filtration, involves the recirculation of the retentate across the surface of the membrane. This gentle cross flow feed acts to minimize membrane fouling, maintain a high filtration rate and provide higher product recovery. Click here for more information about our Single-Use Hollow Fiber Filters.

doi: 10.1016/j.vaccine.2014.09.074