Researchers with the Department of Chemistry and Biochemistry at UCLA wrote “Protein Complexation and pH Dependent Release Using Boronic Acid Containing PEG-Polypeptide Copolymers” for the January 2017 issue of Macromolecular Bioscience. In the paper, the scientists used Spectrum’s RC dialysis tubing to develop a new copolymer as a stimulus responsive drug carrier.
The scientists wanted to create a drug carrier for biological therapeutics that responds to pH or redox. They created a new copolypeptide [poly(l-lysine)-b-poly(ethylene glycol), K8PEG44] to maintain stability but release in certain acidic conditions.
Abstract: New poly(L-lysine)-b-poly(ethylene glycol) copolypeptides have been prepared, where the side-chain amine groups of lysine residues are modified to contain ortho-amine substituted phenylboronic acid, i.e., Wulff-type phenylboronic acid (WBA), groups to improve their pH responsive, carbohydrate binding properties. These block copolymers form nanoscale complexes with glycosylated proteins that are stable at physiological pH, yet dissociate and release the glycoproteins under acidic conditions, similar to those found in endosomal and lysosomal compartments within cells. These results suggest that WBA modified polypeptide copolymers are promising for further development as degradable carriers for intracellular protein delivery. Click here to read the full article.
Large-volume Process Dialysis is an emerging technology driven by the increasing demand for gentle and consistent multi-batch purification at the production scale. While Laboratory Dialysis for research and analytical testing typically involves static stirring of small volumes for sample prep or solute release studies, Process Dialysis facilitates dynamic buffer flow around the membrane-encased sample to increase purification efficiency and improve large buffer handling for the production of fragile proteins, viscous fluids and polymer gels, such as hyaluronic acid.