Researchers with Purdue University wrote “Protein particulate retention and microorganism recovery for rapid detection of Salmonella” in the April 2017 issue of Biotechnology Progress. The scientists used our MidiKros® (D02-P20U-05-N) to concentrate and recover Salmonella cells from turkey homogenate.
The commercial agriculture industry is seeking faster Salmonella and other food-borne illness detection practices. Our hollow fiber filters are used as a second-step microfiltration, after particulate from the turkey homogenate capable of clogging the filter was removed.
The researchers based this experiment on a recent study of Salmonella in egg whites. Egg whites, by nature, do not tend to clog filters. Introducing a more viscous and more particulate-heavy homogenate for Salmonella detection gives the commercial agriculture industry more applications for food-borne illness prevention.
Abstract: The rapid detection of Salmonella in ground meat requires that living microorganisms be brought to levels detectable by PCR, immunoassays, or similar techniques within 8 h. Previously, we employed microfiltration using hollow fiber membranes to rapidly process and concentrate viable bacteria in food extracts through a combination of enzyme treatment and prefiltration in order to prevent blockage or fouling of the hollow fiber membranes. Click here to read the rest of the article.
Polyethersulfone (PES) is hydrophilic membrane chemistry with low protein binding properties. It is more resistant to acids, bases and surfactants than other membrane chemistries.