Scientists from The Ohio State University wrote “Mixtures of tense and relaxed state polymerized human hemoglobin regulate oxygen affinity and tissue construct oxygenation”, published in October 2017 for PLOS One. In the paper, researchers used 0.2 μm, 50 nm, 500 kDa, and 100 kDa for their process.

The scientists analyzed PolyhHb mixtures with different O2 properties for tissue engineering. In this study, the researchers demonstrated their ability to computationally calculate oxygenation of PolyhHb.

Abstract: Pure tense (T) and relaxed (R) quaternary state polymerized human hemoglobins
(PolyhHbs) were synthesized and their biophysical properties characterized, along with
mixtures of T- and R-state PolyhHbs. It was observed that the oxygen affinity of PolyhHb
mixtures varied linearly with T-state mole fraction. Computational analysis of PolyhHb facilitated
oxygenation of a single fiber in a hepatic hollow fiber (HF) bioreactor was performed to
evaluate the oxygenation potential of T- and R-state PolyhHb mixtures. Click here to read the article.


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doi: 10.1371/journal.pone.0185988