Researchers at the University of California, Los Angeles worked with Michael Bransby, the Director of Process Technology at SpectrumLabs.com, to develop a new process for lentiviral vector concentration to replace centrifugation. The article, “Highly efficient large-scale lentiviral vector concentration by tandem tangential flow filtration”, was published in the October 2011 issue of Journal of Virological Methods.
The scientists used the KR2i TFF System and 2 custom flow paths with 500 kDa molecular weight cut off. The flow paths were designed to work in sequence to concentrate the material down to 1 mL with a 97% recovery rate.
Abstract: Large-scale lentiviral vector (LV) concentration can be inefficient and time consuming, often involving multiple rounds of filtration and centrifugation. This report describes a simpler method using two tangential flow filtration (TFF) steps to concentrate liter-scale volumes of LV supernatant, achieving in excess of 2000-fold concentration in less than 3 h with very high recovery (>97%). Click here to read the article.
Spectrum has expanded the concept of disposability by incorporating irradiated KrosFlo hollow fiber filter modules into sterile KrosFlo EZ-Bag Manifolds to provide a totally disposable process filtration flow path. Click here for more information about KrosFlo® MBT Sets.