Researchers with the University of Sonora in Mexico wrote “Performance analysis of exponential-fed perfusion cultures for pDNA vaccines production” in the May 2016 issue of the Journal of Chemical Technology and Biotechnology. The scientists used our KRIIi TFF System and KrosFlo® hollow fiber filter modules for perfusion cultures.

In their analysis, the scientists found that the “higher productivity” of the perfusion bioprocess may be more economically advantageous “compared with the conventional bioprocess”.

Abstract: The clinical application of plasmid DNA (pDNA) vaccines is progressing and it is essential to develop industrial bioprocesses to economically manufacture these macromolecules.  Click here to read the article.

KrosFlo Perfusion Flow-Path Assemblies for bioreactor perfusion are ready-to-use, single-use, and gamma sterilized. With the elimination of autoclaving, setup time is reduced from hours to minutes—in comparison with existing perfusion technologies. The flow paths are scalable from 2L to 2000L working volumes.

The university researchers used our KRIIi system for their perfusion operation, and we recently released our new KR2i TFF System!

The KML [KrosFlo MagLev] Benchtop Perfusion and Tangential Flow Filtration (TFF) System is designed for managing 1.5 L to 100L working volume bioreactors using gamma sterilized single-use perfusion flow-paths (MBT’s). The system uses a single-use, non-pulsating, levitating magnetic pump controlled by the PLC, snd the pump is low shear for improved cell viability. The KML system has a small benchtop footprint and comes with a detachable flow path stand. We created the KML for user friendly perfusion operations and is represented in the above image.

doi: 10.1002/jctb.5011